Human Chorionic Gonadotropin ELISA - Visual HCG pregnancy EIA

Rapid Visual ELISA Test for the Qualitative Determination of Human Chorionic Gonadotropin (hCG) in Urine or Serum

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES

SUMMARY
Human chorionic gonadotropin can be used as a tumor marker, as its ? subunit is secreted by some cancers including seminoma, choriocarcinoma, germ cell tumors, hydatidiform mole formation, teratoma with elements of choriocarcinoma, and islet cell tumor. For this reason a positive result in males can be a test for testicular cancer. The normal range for men is between 0-5 mIU/mL. Combined with alpha-fetoprotein, ?-HCG is an excellent tumor marker for the monitoring of germ cell tumors.

PRINCIPLE OF THE TEST
The Visual hCG ELISA Test is a sandwich enzyme immunoassay for the determination of human chorionic gonadotropin in urine or serum. The method employs two monoclonal antibodies to selectively identify hCG in urine/serum with a high degree of sensitivity. In less than 10 minutes, elevated levels of hCG as little as 20 mIU/ml can be detected. The patient’s specimen is allowed to react with the antibody enzyme conjugate and the antibodies on the solid phase simultaneously. In the presence of hCG, a specific antibody-hCG-antibody-enzyme complex will form on the surface of microtiter well. After unbound enzyme conjugate is removed by rinsing under a stream of distilled water, the well is incubated with TMB Reagent. The development of blue color in the well indicated the presence of hCG.Comparing the color intensity of patient samples with that of the provided known reference, the amount of hCG can be visually estimated to be greater or less than 20 mIU/ml.