Categories

Human Chorionic Gonadotropin ELISA - Free Beta-HCG

Human Chorionic Gonadotropin ELISA - Free Beta-HCG
Human Chorionic Gonadotropin ELISA - Free Beta-HCG
Product Code: HCG-E-B01
Availability: In Stock
Price: $220.00
Qty:     - OR -   Add to Favorites List
Add to Compare

Enzyme Immunoassay for the Determination of Free Beta-Subunit of Chorionic Gonadotropin (Free Beta-hCG) in Serum

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES

SUMMARY
Human chorionic gonadotropin can be used as a tumor marker, as its ? subunit is secreted by some cancers including seminoma, choriocarcinoma, germ cell tumors, hydatidiform mole formation, teratoma with elements of choriocarcinoma, and islet cell tumor. For this reason a positive result in males can be a test for testicular cancer. The normal range for men is between 0-5 mIU/mL. Combined with alpha-fetoprotein, ?-HCG is an excellent tumor marker for the monitoring of germ cell tumors.

PRINCIPLE OF THE TEST
The free Beta-hCG ELISA test is based on the principle of a solid phase enzyme-linked immunosorbent assay.1 The assay system utilizes a unique monoclonal antibody directed against a distinct antigenic determinant on the free Beta-hCG. Mouse monoclonal anti-free-Beta-hCG antibody is used for solid phase immobilization (on the microtiter wells). A goat anti whole hCG antibody is in the antibody-enzyme (horseradish peroxidase) conjugate solution. The test sample is allowed to react sequentially with the two antibodies, resulting in the free Beta-hCG molecules being sandwiched between the solid phase and enzyme-linked antibodies. After two separate 30 minute incubations at 37 °C, the wells are washed with water to remove unbound labeled antibodies. A solution of TMB Reagent is added and incubated for 20 minutes, resulting in the development of a blue color. The color development is stopped with the addition of Stop Solution changing the color to yellow. The concentration of Beta-hCG is directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm.

EXAMPLE OF STANDARD CURVE
Results of a typical standard run with optical density readings at 450 nm shown in the Y axis against Human Chorionic Gonadotropin concentrations shown in the X axis. This standard curve is for the purpose of illustration only, and should not be used to calculate unknowns. Each user should obtain his or her own data and standard curve.

General
ANALYTE GROUP Human Chorionic Gonadotropin
GROUPING Hormones:Reproduction
PRODUCT NAME ELISA
STORAGE Store the kit at 2-8°C
COUNTRY OF ORIGIN USA
DISCOUNTS Bulk Packaging; High Volume
ELISA
TESTS PER KIT 96 (12 x 8)
CALIBRATION RANGE 0 - 50 ng/mL (Recommended)
  • Amphetamines
  • Cocaine
  • Methaqualone
  • Lysergic Acid Diethylamide
  • Methadone
  • Methamphetamines
  • Carisoprodol
  • Clonazepam
  • Oxycodone
  • Phenobarbitol
  • Morphine
  • Propoxyphene
  • Cannabinoids
  • Phencyclidine
  • Tapentadol
  • Tricyclics
  • Secobarbitol
  • Zolpidem
  • MDMA
  • Fentanyl
  • Acetominophen
  • Benzodiazepines
  • Tramadol
  • K2 (Spice)
  • Salicylate
  • Buprenorphine
  • Barbituates
  • Ketamine

United Immunoassay, Inc. © 2018